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Journal of Clinical Microbiology, February 2009, p. 481-484, Vol. 47, No. 2
0095-1137/09/$08.00+0     doi:10.1128/JCM.01253-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Evaluation of an Immunochromatographic Assay Kit for Rapid Identification of Mycobacterium tuberculosis Complex in Clinical Isolates{triangledown}

Mi Young Park,1* Young Jin Kim,1 Sang Hyun Hwang,1 Hyoung Hoi Kim,1 Eun Yup Lee,1 Seok Hoon Jeong,2 and Chulhun L. Chang1,3

Department of Laboratory Medicine, School of Medicine,1 Medical Research Institute, Pusan National University, Busan,3 Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea2

Received 2 July 2008/ Returned for modification 16 August 2008/ Accepted 22 November 2008

We evaluated a new immunochromatographic assay (ICA) using mouse monoclonal anti-MPT64 antibody for rapid discrimination between Mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. A study with mycobacteria and other organisms showed excellent sensitivity ({cong}99%) and specificity (100%) and an appropriate detection limit (105 CFU/ml) when tested with M. tuberculosis H37Rv. This ICA can simplify the identification of M. tuberculosis in clinical laboratories.

* Corresponding author. Mailing address: Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan 602-739, Korea. Phone: 82-51-240-7417. Fax: 82-51-247-6560. E-mail: CCHL{at}pusan.ac.kr

{triangledown} Published ahead of print on 3 December 2008.

Journal of Clinical Microbiology, February 2009, p. 481-484, Vol. 47, No. 2
0095-1137/09/$08.00+0     doi:10.1128/JCM.01253-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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