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Journal of Clinical Microbiology, February 2009, p. 489-491, Vol. 47, No. 2
0095-1137/09/$08.00+0     doi:10.1128/JCM.01612-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Comparison of Neisseria gonorrhoeae Multiantigen Sequence Typing and porB Sequence Analysis for Identification of Clusters of N. gonorrhoeae Isolates{triangledown} ,{dagger}

Mingmin Liao,1,2 Sarah Helgeson,2,3 Wei-Ming Gu,4 Yang Yang,4 Ann M. Jolly,5 and Jo-Anne R. Dillon1,2,3*

Department of Microbiology and Immunology,1 Vaccine and Infectious Disease Organization,2 Department of Biology, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada,3 Shanghai Skin Disease and STD Hospital, 196 Wu Yi Road, Shanghai, China 200050,4 Department of Epidemiology and Community Medicine, Faculty of Medicine, University of Ottawa, Roger-Guindon Hall, 451 Smyth Road, Ottawa, Ontario K1H 8M5, Canada5

Received 19 August 2008/ Returned for modification 14 October 2008/ Accepted 24 November 2008

porB DNA sequence analysis and Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) methods were compared for their abilities to discriminate strains and to identify epidemiologically congruent pairs of N. gonorrhoeae. Both methods provided high-level discrimination of strains. NG-MAST further differentiated large porB-based clusters. However, considerations of cost suggest that porB DNA sequence analysis is a useful tool for preliminary molecular analysis of the epidemiology of N. gonorrhoeae.

* Corresponding author. Mailing address: Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada. Phone: (306) 966-4232. Fax: (306) 966-8839. E-mail: j.dillon{at}usask.ca

{triangledown} Published ahead of print on 3 December 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, February 2009, p. 489-491, Vol. 47, No. 2
0095-1137/09/$08.00+0     doi:10.1128/JCM.01612-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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