This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liao, R. S.
Right arrow Articles by Zoutman, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liao, R. S.
Right arrow Articles by Zoutman, D. E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2009, p. 527-532, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01213-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Comparison of Viral Isolation and Multiplex Real-Time Reverse Transcription-PCR for Confirmation of Respiratory Syncytial Virus and Influenza Virus Detection by Antigen Immunoassays{triangledown}

R. S. Liao,1,2* L. L. Tomalty,1,2 A. Majury,2 and D. E. Zoutman1,2

Division of Medical Microbiology and Infection Control, Department of Pathology and Molecular Medicine,1 Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada2

Received 26 June 2008/ Returned for modification 24 September 2008/ Accepted 27 December 2008

We evaluated the Prodesse ProFlu-1 real-time reverse transcription-PCR multiplex assay with the SmartCycler instrument for the detection of human respiratory syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassays with the BD Directigen A+B and Binax NOW RSV assays over two successive respiratory virus seasons. Ninety-two percent of the 361 specimens tested were nasopharyngeal aspirates obtained from individual patients, of which 119 were positive for RSV and 59 were positive for influenza virus. The median age of the patients whose specimens were positive for RSV and influenza virus were 6.3 months and 42.4 years, respectively. The specificity of all of the methods tested was ≥99%, and the individual sensitivities of NOW RSV, RSV culture, Directigen A+B, influenza virus culture, and the Proflu-1 PCR for influenza/RSV were 82% (95% confidence interval [CI], 73 to 88), 57% (95% CI, 44 to 69), 59% (95% CI, 44 to 72), 54% (95% CI, 38 to 69), and 98% (95% CI, 93 to 100)/95% (95% CI, 85 to 99), respectively. In a clinical setting where viral isolation is performed to confirm rapid antigen immunoassay results for these common respiratory viruses, one-step real-time reverse transcriptase PCR testing can be a more sensitive and timely confirmatory method.

* Corresponding author. Mailing address: Division of Medical Microbiology and Infection Control, Department of Pathology and Molecular Medicine, Kingston General Hospital, Douglas 3, Room 315, 76 Stuart Street, Kingston, Ontario K7L 2V7, Canada. Phone: (613) 549-6666, ext. 3272. Fax: (613) 548-2513. E-mail: robertliaocardinals{at}hotmail.com

{triangledown} Published ahead of print on 7 January 2009.

Journal of Clinical Microbiology, March 2009, p. 527-532, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01213-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Agrawal, A. S., Sarkar, M., Chakrabarti, S., Rajendran, K., Kaur, H., Mishra, A. C., Chatterjee, M. K., Naik, T. N., Chadha, M. S., Chawla-Sarkar, M. (2009). Comparative evaluation of real-time PCR and conventional RT-PCR during a 2 year surveillance for influenza and respiratory syncytial virus among children with acute respiratory infections in Kolkata, India, reveals a distinct seasonality of infection. J Med Microbiol 58: 1616-1622 [Abstract] [Full Text]  
  • Mahony, J. B., Blackhouse, G., Babwah, J., Smieja, M., Buracond, S., Chong, S., Ciccotelli, W., O'Shea, T., Alnakhli, D., Griffiths-Turner, M., Goeree, R. (2009). Cost Analysis of Multiplex PCR Testing for Diagnosing Respiratory Virus Infections. J. Clin. Microbiol. 47: 2812-2817 [Abstract] [Full Text]  
  • Bose, M. E., Beck, E. T., Ledeboer, N., Kehl, S. C., Jurgens, L. A., Patitucci, T., Witt, L., LaGue, E., Darga, P., He, J., Fan, J., Kumar, S., Henrickson, K. J. (2009). Rapid Semiautomated Subtyping of Influenza Virus Species during the 2009 Swine Origin Influenza A H1N1 Virus Epidemic in Milwaukee, Wisconsin. J. Clin. Microbiol. 47: 2779-2786 [Abstract] [Full Text]  
  • He, J., Bose, M. E., Beck, E. T., Fan, J., Tiwari, S., Metallo, J., Jurgens, L. A., Kehl, S. C., Ledeboer, N., Kumar, S., Weisburg, W., Henrickson, K. J. (2009). Rapid Multiplex Reverse Transcription-PCR Typing of Influenza A and B Virus, and Subtyping of Influenza A Virus into H1, 2, 3, 5, 7, 9, N1 (Human), N1 (Animal), N2, and N7, Including Typing of Novel Swine Origin Influenza A (H1N1) Virus, during the 2009 Outbreak in Milwaukee, Wisconsin. J. Clin. Microbiol. 47: 2772-2778 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»