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Journal of Clinical Microbiology, March 2009, p. 711-714, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01712-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Use of the BacT/Alert MB Mycobacterial Blood Culture System for Detection of Mycobacteria in Sterile Body Fluids Other than Blood{triangledown}

Romano Mattei,1* Arnaldo Savarino,1 Marco Fabbri,2 Sara Moneta,3 and Enrico Tortoli4

Clinical Laboratory,1 Blood Bank,2 Department of Infectious Diseases, Campo di Marte Hospital, Lucca 55100,3 Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, Florence 50134, Italy4

Received 4 September 2008/ Returned for modification 21 October 2008/ Accepted 14 December 2008

The definitive diagnosis of extrapulmonary tuberculosis is made by a positive body fluid culture result. Conventional culture methods require centrifugation or filtration of body fluid (peritoneal, pleural, synovial, or pericardial fluid) in order to improve the sensitivity. The aim of the present study was to evaluate the feasibility of the direct inoculation, at the patient's bedside, of up to 5 ml of uncentrifuged fluid onto BacT/Alert MB culture bottles (bioMérieux, Durham, NC).

* Corresponding author. Mailing address: Clinical Laboratory, Campo di Marte Hospital, Lucca 55100, Italy. Phone: 39 0583970313. Fax: 39 0583970402. E-mail: r.mattei{at}usl2.toscana.it

{triangledown} Published ahead of print on 24 December 2008.

Journal of Clinical Microbiology, March 2009, p. 711-714, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01712-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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