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Journal of Clinical Microbiology, March 2009, p. 715-720, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.00747-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genetic Analysis of Group A Streptococcus Isolates Recovered during Acute Glomerulonephritis Outbreaks in Guizhou Province of China{triangledown}

Ming-huan Zheng,1 Zhen-quan Jiao,2 Li-jie Zhang,3 Sang-jie Yu,4 Guang-peng Tang,5 Xiao-mei Yan,1 Li-hua He,1 Fan-liang Meng,1 Fei Zhao,1 Mao-jun Zhang,1 Di Xiao,1 Yong-hong Yang,4 Wei Nie,5 Jian-zhong Zhang,1* and Zi-jun Wang2*

Department of Diagnosis, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China,1 Office of Disease Control and Emergency Response, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China,2 CFETP, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China,3 Beijing Children's Hospital Affiliated to Capital Medical University, Beijing, People's Republic of China,4 Center for Disease Control and Prevention of Guizhou Province, Guiyang, People's Republic of China5

Received 19 April 2008/ Returned for modification 18 August 2008/ Accepted 22 December 2008

In this study, 68 group A streptococcus (GAS) isolates associated with two outbreaks of acute glomerulonephritis (AGN) in China were analyzed by emm typing. A total of 11 different emm types were identified. Analysis of emm type distribution suggested that AGN outbreaks in two counties were caused by emm60.1- and emm63.0-type GAS. These two types were further characterized by pulsed-field gel electrophoresis, multilocus sequence typing, sof sequence typing, and PCR-based identification of streptococcal pyrogenic exotoxin A, B, and C (speA, speB, and speC) genes. In antimicrobial susceptibility tests, all outbreak strains were resistant to erythromycin and tetracycline, and the rates of resistance of nonoutbreak strains to the two antibiotics were 63.6% and 90.9%. This study is also the first to report a nephritogenic M63 GAS strain.

* Corresponding author. Mailing address for Jian-zhong Zhang: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, P.O. Box 5, Changping, Beijing 102206, People's Republic of China. Phone and fax: 86-10-61739456. E-mail: helico99{at}sina.com. Mailing address for Zi-jun Wang: Office of Disease Control and Emergency Response, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China. Phone: 86-13-910751827. Fax: 86-10-63152572. E-mail: wangzj{at}chinacdc.cn

{triangledown} Published ahead of print on 30 December 2008.

Journal of Clinical Microbiology, March 2009, p. 715-720, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.00747-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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