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Journal of Clinical Microbiology, March 2009, p. 743-750, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01297-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Comparison of Automated Microarray Detection with Real-Time PCR Assays for Detection of Respiratory Viruses in Specimens Obtained from Children{triangledown}

Frédéric Raymond,1 Julie Carbonneau,1 Nancy Boucher,1 Lynda Robitaille,1 Sébastien Boisvert,1 Whei-Kuo Wu,2 Gaston De Serres,3 Guy Boivin,1* and Jacques Corbeil1*

Infectious Disease Research Center of the CHUQ-CHUL and Laval University, Saint-Foy, Quebec, Canada,1 AutoGenomics, Inc., Carlsbad, California,2 Institut National de Santé Publique du Québec, Quebec City, Quebec, Canada3

Received 9 July 2008/ Returned for modification 3 October 2008/ Accepted 9 January 2009

Respiratory virus infections are a major health concern and represent the primary cause of testing consultation and hospitalization for young children. We developed and compared two assays that allow the detection of up to 23 different respiratory viruses that frequently infect children. The first method consisted of single TaqMan quantitative real-time PCR assays in a 96-well-plate format. The second consisted of a multiplex PCR followed by primer extension and microarray hybridization in an integrated molecular diagnostic device, the Infiniti analyzer. Both of our assays can detect adenoviruses of groups A, B, C, and E; coronaviruses HKU1, 229E, NL63, and OC43; enteroviruses A, B, C, and D; rhinoviruses of genotypes A and B; influenza viruses A and B; human metapneumoviruses (HMPV) A and B, human respiratory syncytial viruses (HRSV) A and B; and parainfluenza viruses of types 1, 2, and 3. These tests were used to identify viruses in 221 nasopharyngeal aspirates obtained from children hospitalized for respiratory tract infections. Respiratory viruses were detected with at least one of the two methods in 81.4% of the 221 specimens: 10.0% were positive for HRSV A, 38.0% for HRSV B, 13.1% for influenzavirus A, 8.6% for any coronaviruses, 13.1% for rhinoviruses or enteroviruses, 7.2% for adenoviruses, 4.1% for HMPV, and 1.5% for parainfluenzaviruses. Multiple viral infections were found in 13.1% of the specimens. The two methods yielded concordant results for 94.1% of specimens. These tests allowed a thorough etiological assessment of respiratory viruses infecting children in hospital settings and would assist public health interventions.

* Corresponding author. Mailing address for G. Boivin and J. Corbeil: Infectious Disease Research Center of the CHUQ-CHUL and Laval University, 2705, Boulevard Laurier, Sainte-Foy (Quebec), G1V 4G2 Canada. Phone: 418-654-2705. Fax: 418-654-2715. E-mail for G. Boivin: guy.boivin{at}crchul.ulaval.ca. E-mail for J. Corbeil: jacques.corbeil{at}crchul.ulaval.ca

{triangledown} Published ahead of print on 21 January 2009.

Journal of Clinical Microbiology, March 2009, p. 743-750, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01297-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Miller, M. B., Tang, Y.-W. (2009). Basic Concepts of Microarrays and Potential Applications in Clinical Microbiology. Clin. Microbiol. Rev. 22: 611-633 [Abstract] [Full Text]  


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