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Journal of Clinical Microbiology, March 2009, p. 781-784, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01645-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Melting Curve Analysis for Rapid Detection of Topoisomerase Gene Mutations in Haemophilus influenzae{triangledown}

Shigeki Nakamura,1 Katsunori Yanagihara,1,2* Yoshitomo Morinaga,1,2 Koichi Izumikawa,1 Masafumi Seki,1 Hiroshi Kakeya,1 Yoshihiro Yamamoto,1 Shimeru Kamihira,2 and Shigeru Kohno1,3

Second Department of Internal Medicine,1 Department of Laboratory Medicine,2 Division of Molecular and Clinical Microbiology, Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan3

Received 24 August 2008/ Returned for modification 5 December 2008/ Accepted 22 December 2008

We established a real-time PCR assay with melting curve analysis to rapidly genotype quinolone resistance-determining regions (QRDRs) of gyrase A and topoisomerase IV genes in Haemophilus influenzae. This assay is a useful tool for the detection of fluoroquinolone resistance and for the early detection of preexisting QRDR mutations.

* Corresponding author. Mailing address: Department of Laboratory Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan. Phone: 81-95-819-7276. Fax: 81-95-849-7285. E-mail: k-yanagi{at}nagasaki-u.ac.jp

{triangledown} Published ahead of print on 7 January 2009.

Journal of Clinical Microbiology, March 2009, p. 781-784, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01645-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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