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Journal of Clinical Microbiology, March 2009, p. 827-829, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.02464-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

High Concentrations of Manganese in Mueller-Hinton Agar Increase MICs of Tigecycline Determined by Etest{triangledown}

Carlos Fernández-Mazarrasa,1 Olav Mazarrasa,2 Jorge Calvo,1 Asunción del Arco,1 and Luis Martínez-Martínez1,3*

Service of Microbiology, University Hospital Marqués de Valdecilla,1 Department of Molecular Biology, School of Medicine,3 University of Cantabria, and Occupational Safety and Health Center, Santander, Spain2

Received 22 December 2008/ Accepted 2 January 2009

MICs of tigecycline determined by Etest were 4 to 12 times (three ATCC strains) and 2 to 8 times (50 clinical isolates) higher in Mueller-Hinton agar from Merck than in Mueller-Hinton agar from either Oxoid or Difco. This was related to a much higher concentration of manganese in the medium from Merck.

* Corresponding author. Mailing address: Service of Microbiology, Hospital Universitario Marqués de Valdecilla, Avda. de Valdecilla s/n, Santander 39008, Spain. Phone: 34 942 202580. Fax: 34 942 203462. E-mail: lmartinez{at}humv.es

{triangledown} Published ahead of print on 14 January 2009.

Journal of Clinical Microbiology, March 2009, p. 827-829, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.02464-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



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