This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fang, R.
Right arrow Articles by Gao, Q.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fang, R.
Right arrow Articles by Gao, Q.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2009, p. 845-847, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01528-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Cross-Priming Amplification for Rapid Detection of Mycobacterium tuberculosis in Sputum Specimens{triangledown}

Rendong Fang,1,2 Xia Li,2 Lin Hu,3 Qimin You,3 Jing Li,1 Jie Wu,1 Peng Xu,1,2 Huayan Zhong,3 Ying Luo,3 Jian Mei,1 and Qian Gao2*

Department of TB Control, Shanghai Municipal Centers for Disease Control and Prevention, Shanghai 200336, China,1 Key Laboratory of Medical Molecular Virology, Institutes of Biomedical Sciences and Institute of Medical Microbiology, Fudan University, Shanghai 200032, China,2 Ustar Biotechnologies (Hangzhou) Co., Ltd., Hangzhou, Zhejiang 310012, China3

Received 8 August 2008/ Returned for modification 1 October 2008/ Accepted 22 December 2008

Cross-priming amplification (CPA) technology for tuberculosis diagnosis from sputum specimens was evaluated. The sensitivity of CPA from smear- and liquid culture-positive specimens was 96.9%, and that from smear-negative and liquid culture-positive specimens was 87.5%. The specificity of CPA in culture-negative specimens was 98.8%.

* Corresponding author. Mailing address: Shanghai Medical College, Fudan University, 138 Yi Xue Yuan Road, Shanghai, China 200032. Phone: (8621) 5423-7195. Fax: (8621) 5423-7971. E-mail: qgao99{at}yahoo.com

{triangledown} Published ahead of print on 30 December 2008.

Journal of Clinical Microbiology, March 2009, p. 845-847, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01528-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»