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Journal of Clinical Microbiology, April 2009, p. 1082-1086, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.01850-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification of Staphylococcus Species Directly from Positive Blood Culture Broth by Use of Molecular and Conventional Methods{triangledown}

Maitry S. Mehta,1* Suzanne M. Paule,1 Richard B. Thomson,1,2 Karen L. Kaul,1,2 and Lance R. Peterson1,2

NorthShore University HealthSystem, Evanston, Illinois,1 Feinberg School of Medicine, Northwestern University, Chicago, Illinois2

Received 24 September 2008/ Returned for modification 14 November 2008/ Accepted 5 February 2009

We compared two real-time PCR assays (both by the use of melting curve analysis) for their ability to identify Staphylococcus species directly from 200 positive blood culture bottles. The PCR assays correctly identified 83% to 94% of the Staphylococcus isolates to species clusters. Molecular testing significantly outperformed commercially available latex tests (sensitivity for both latex tests, <15%) when it was used directly with broth from signal-positive blood cultures.

* Corresponding author. Mailing address: NorthShore University HealthSystem, 2650 Ridge Avenue, Walgreen Building Rm. #SB 525, Evanston, IL 60201. Phone: (847) 570-1790. Fax: (847) 733-5093. E-mail: mmehta{at}northshore.org

{triangledown} Published ahead of print on 11 February 2009.

Journal of Clinical Microbiology, April 2009, p. 1082-1086, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.01850-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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