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Journal of Clinical Microbiology, April 2009, p. 1212-1215, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.02265-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Using a Commercial DiversiLab Semiautomated Repetitive Sequence-Based PCR Typing Technique for Identification of Escherichia coli Clone ST131 Producing CTX-M-15{triangledown}

Johann D. D. Pitout,1,2,4* Lorraine Campbell,1 Deirdre L. Church,1,2,3 Pauline W. Wang,5 David S. Guttman,5 and Daniel B. Gregson1,2,3

Division of Microbiology, Calgary Laboratory Services, Calgary,1 Departments of Pathology and Laboratory Medicine,2 Medicine,3 Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada,4 Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario, Canada5

Received 25 November 2008/ Returned for modification 7 January 2009/ Accepted 2 February 2009

A study was designed to evaluate the ability of the DiversiLab fingerprinting kit, a type of repetitive element PCR (rep-PCR), to identify Escherichia coli clone ST131 producing β-lactamase CTX-M-15. A set of 53 nonduplicate isolates of extended-spectrum β-lactamase-producing E. coli underwent rep-PCR, pulsed-field gel electrophoresis, and multilocus sequence typing. The DiversiLab system successfully identified E. coli clone ST131 producing CTX-M-15 and provides a simple standardized typing protocol for monitoring the spread of this clone.

* Corresponding author. Mailing address: Calgary Laboratory Services, Building 9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8. Phone: (403) 770-3309. Fax: (403) 770-3347. E-mail: johann.pitout{at}cls.ab.ca

{triangledown} Published ahead of print on 9 February 2009.

Journal of Clinical Microbiology, April 2009, p. 1212-1215, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.02265-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



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  • Pitout, J. D. D., Gregson, D. B., Campbell, L., Laupland, K. B. (2009). Molecular Characteristics of Extended-Spectrum-{beta}-Lactamase-Producing Escherichia coli Isolates Causing Bacteremia in the Calgary Health Region from 2000 to 2007: Emergence of Clone ST131 as a Cause of Community-Acquired Infections. Antimicrob. Agents Chemother. 53: 2846-2851 [Abstract] [Full Text]  


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