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Journal of Clinical Microbiology, April 2009, p. 1238-1240, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.02204-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Single-Point Mutations Causing More than 100-Fold Underestimation of Human Immunodeficiency Virus Type 1 (HIV-1) Load with the Cobas TaqMan HIV-1 Real-Time PCR Assay{triangledown}

Klaus Korn,1* Benedikt Weissbrich,2 Cornelia Henke-Gendo,3 Albert Heim,3 Christin Mariel Jauer,4 Ninon Taylor,5 and Josef Eberle4

Institute of Clinical and Molecular Virology, National Reference Center for Retroviruses, University of Erlangen-Nuremberg, Erlangen, Germany,1 Institute of Virology and Immunobiology, University of Würzburg, Würzburg, Germany,2 Institute of Virology, Medical School Hannover, Hannover, Germany,3 Max von Pettenkofer-Institute, Ludwig-Maximilians-University, Munich, Germany,4 Third Medical Department, Paracelsus Medical University of Salzburg, Salzburg, Austria5

Received 18 November 2008/ Accepted 26 January 2009

Systematic sequence analysis of human immunodeficiency virus type 1 (HIV-1) variants with RNA levels underestimated by the Cobas TaqMan HIV-1 assay demonstrated that mutations at a single position of the downstream primer can lead to the underestimation of HIV-1 RNA levels by more than 2 log and to false-negative results in minipool screening of blood donors. Mutations at this position are found in about 2% of all HIV-1 M gag sequences.

* Corresponding author. Mailing address: Institute of Clinical and Molecular Virology, National Reference Center for Retroviruses, University of Erlangen-Nuremberg, Schlossgarten 4, 91054 Erlangen, Germany. Phone: 49-9131-8524010. Fax: 49-9131-8526485. E-mail: klaus.korn{at}viro.med.uni-erlangen.de

{triangledown} Published ahead of print on 4 February 2009.

Journal of Clinical Microbiology, April 2009, p. 1238-1240, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.02204-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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