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Journal of Clinical Microbiology, April 2009, p. 908-913, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.01966-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Eight-Plex PCR and Liquid-Array Detection of Bacterial and Viral Pathogens in Cerebrospinal Fluid from Patients with Suspected Meningitis{triangledown}

Mette Kusk Bøving, Lisbeth Nørum Pedersen, and Jens Kjølseth Møller*

Department of Clinical Microbiology, Aarhus University Hospital, Skejby, Århus N DK-8200, Denmark

Received 11 October 2008/ Returned for modification 20 November 2008/ Accepted 22 January 2009

We here report on the development of a novel multiplex PCR with product detection in a Luminex 100 suspension array system. The assay covers the nine most important bacterial and viral pathogens found in Danish meningitis patients. The microorganisms include Neisseria meningitidis, Streptococcus pneumoniae, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Streptococcus agalactiae, herpes simplex virus types 1 and 2, and varicella-zoster virus. The study was based on 1,187 samples, of which 55 were found to be positive by PCR. The assay was found to have an excellent sensitivity and an excellent specificity compared to the results of a "gold standard," defined by routine laboratory tests, for the two most important pathogens, S. pneumoniae (95 and 99.1%, respectively) and N. meningitidis (100 and 99.7%, respectively). The method provides a valuable supplement to the traditional microscopy and culture of cerebrospinal fluid (CSF) samples in a routine diagnostic setting, and results can be available within 1 workday. The method is suitable for use for the initial screening and identification of nine important microorganisms in CSF samples from patients with suspected meningitis. Compared to microscopy and culture of CSF, this rapid and sensitive method will support physicians with the selection of the appropriate antimicrobial agents and the initiation of timely treatment in the absence of live microorganisms in the CSF.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Aarhus University Hospital, Brendstrupgaardsvej 100, Skejby, Århus N DK-8200, Denmark. Phone: 45 8949 5609. Fax: 45 8949 5611. E-mail: jkm{at}dadlnet.dk

{triangledown} Published ahead of print on 4 February 2009.

Journal of Clinical Microbiology, April 2009, p. 908-913, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.01966-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Miller, M. B., Tang, Y.-W. (2009). Basic Concepts of Microarrays and Potential Applications in Clinical Microbiology. Clin. Microbiol. Rev. 22: 611-633 [Abstract] [Full Text]  


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