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Journal of Clinical Microbiology, May 2009, p. 1546-1548, Vol. 47, No. 5
0095-1137/09/$08.00+0     doi:10.1128/JCM.00114-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Automation of the BD GeneOhm Methicillin-Resistant Staphylococcus aureus Assay for High-Throughput Screening of Nasal Swab Specimens{triangledown}

Xue-Ping Wang and Christine C. Ginocchio*

Molecular Diagnostics, Department of Laboratory Medicine, North Shore—LIJ Health System Laboratories, 10 Nevada Drive, Lake Success, New York 11042

Received 19 January 2009/ Returned for modification 1 March 2009/ Accepted 9 March 2009

This study demonstrated that an automated version of the BD-GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) assay (BD-MRSA), using achromopeptidase sample lysis and PCR setup performed on the Hamilton MICROLAB STARlet (Auto-MRSA), gave results comparable to those obtained with BD-MRSA. The positive- and negative-result concordance rates and overall concordance of BD-MRSA and Auto-MRSA were 98.2, 97.7, and 97.6%, respectively. Auto-MRSA required 60% less technical time than BD-MRSA, and the time required to obtain results was 50% of that required for BD-MRSA. Auto-MRSA provides a reliable, robust system for high-volume clinical use.

* Corresponding author. Mailing address: North Shore—LIJ Health System Laboratories, 10 Nevada Drive, Lake Success, NY 11042. Phone: (516) 719-1079. Fax: (516) 719-1254. E-mail: CGinocch{at}nshs.edu

{triangledown} Published ahead of print on 18 March 2009.

Journal of Clinical Microbiology, May 2009, p. 1546-1548, Vol. 47, No. 5
0095-1137/09/$08.00+0     doi:10.1128/JCM.00114-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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