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Journal of Clinical Microbiology, May 2009, p. 1562-1564, Vol. 47, No. 5
0095-1137/09/$08.00+0     doi:10.1128/JCM.00124-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Interlaboratory Reproducibility of a Single-Locus Sequence-Based Method for Strain Typing of Aspergillus fumigatus{triangledown}

S. F. Hurst,1 S. E. Kidd,2 C. O. Morrissey,2 E. Snelders,3 W. J. G. Melchers,3 M. V. Castelli,4 E. Mellado,4 K. Simmon,5 C. A. Petti,5 S. Richardson,6 S. Zhang,6 A. M. Romanelli,7 B. L. Wickes,7 H. A. de Valk,8 C. H. W. Klaassen,8 and S. Arunmozhi Balajee1*

Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia,1 Department of Medicine, Monash University, Central and Eastern Clinical School, Alfred Hospital, Melbourne, VIC, Australia,2 Department of Medical Microbiology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands,3 MadSpain Servicio de Micologia, Centro Nacional de Microbiologia Instituto de Salud Carlos IIrid, Majadahonda, Madrid, Spain,4 ARUP Laboratories, Salt Lake City, Utah,5 Ontario Ministry of Health, Public Health Laboratories Branch, Toronto, Canada,6 the University of Texas Health Science Center at San Antonio, San Antonio, Texas,7 Canisius Wilhelmina Hospital, Nijmegen, The Netherlands8

Received 21 January 2009/ Returned for modification 13 February 2009/ Accepted 22 February 2009

Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

* Corresponding author. Mailing address: Mycotic Diseases Branch, Centers for Disease Control and Prevention, Mail Stop G 11, 1600 Clifton Road, Atlanta, GA 30333. Phone: (404) 639-3337. Fax: (404) 639-3546. E-mail: fir3{at}cdc.gov

{triangledown} Published ahead of print on 4 March 2009.

Journal of Clinical Microbiology, May 2009, p. 1562-1564, Vol. 47, No. 5
0095-1137/09/$08.00+0     doi:10.1128/JCM.00124-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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