This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Google Scholar Articles by Hall, T. A. Articles by Carroll, K. C. PubMed PubMed Citation Articles by Hall, T. A. Articles by Carroll, K. C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2009, p. 1733-1741, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.02175-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Rapid Molecular Genotyping and Clonal Complex Assignment of Staphylococcus aureus Isolates by PCR Coupled to Electrospray Ionization-Mass Spectrometry

Thomas A. Hall,¹ Rangarajan Sampath,¹ Lawrence B. Blyn,¹ Raymond Ranken,¹ Cristina Ivy,¹ Rachael Melton,¹ Heather Matthews,¹ Neill White,¹ Feng Li,¹ Vanessa Harpin,¹ David J. Ecker,¹ Linda K. McDougal,² Brandi Limbago,² Tracy Ross,⁴ Donna M. Wolk,³ Vicki Wysocki,³ and Karen C. Carroll^4*

Ibis Biosciences, Inc., 1896 Rutherford Rd., Carlsbad, California 92008,¹ Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, 1600 Clifton Rd., Atlanta, Georgia 30333,² University of Arizona, 1501 N. Campbell Ave., Tucson, Arizona,³ Johns Hopkins University, 600 N. Wolfe Street, Baltimore, Maryland 21287⁴

Received 12 November 2008/ Returned for modification 6 January 2009/ Accepted 10 March 2009

We describe a high-throughput assay using PCR coupled to electrospray ionization-mass spectrometry (PCR/ESI-MS) to determine the genotypes of Staphylococcus aureus isolates. The primer sets used in the PCR/ESI-MS assay were designed to amplify the same genes analyzed in multilocus sequence typing (MLST). The method was used to identify the clonal complex and USA type of each isolate and is suitable for use in a clinical or public-health setting. The method was validated using a panel of diverse isolates from the Centers for Disease Control and Prevention that were previously characterized by MLST and pulsed-field gel electrophoresis (PFGE). Clinical isolates from two geographically distinct hospitals were characterized, and the clustering results were in agreement with those for repetitive-element PCR and PFGE. The PCR/ESI-MS method enables genotyping of over 180 samples of S. aureus per day in an automated fashion.

---

* Corresponding author. Mailing address: Johns Hopkins Hospital, 600 N. Wolfe Street, Baltimore, MD 21287. Phone: (410) 955-5077. Fax: (410) 614-8087. E-mail: kcarrol7{at}jhmi.edu

Published ahead of print on 18 March 2009.

---

Journal of Clinical Microbiology, June 2009, p. 1733-1741, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.02175-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Wolk, D. M., Blyn, L. B., Hall, T. A., Sampath, R., Ranken, R., Ivy, C., Melton, R., Matthews, H., White, N., Li, F., Harpin, V., Ecker, D. J., Limbago, B., McDougal, L. K., Wysocki, V. H., Cai, M., Carroll, K. C. (2009). Pathogen Profiling: Rapid Molecular Characterization of Staphylococcus aureus by PCR/Electrospray Ionization-Mass Spectrometry and Correlation with Phenotype. J. Clin. Microbiol. 47: 3129-3137 [Abstract] [Full Text]  
• Ruimy, R., Armand-Lefevre, L., Barbier, F., Ruppe, E., Cocojaru, R., Mesli, Y., Maiga, A., Benkalfat, M., Benchouk, S., Hassaine, H., Dufourcq, J.-B., Nareth, C., Sarthou, J.-L., Andremont, A., Feil, E. J. (2009). Comparisons between Geographically Diverse Samples of Carried Staphylococcus aureus. J. Bacteriol. 191: 5577-5583 [Abstract] [Full Text]