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Journal of Clinical Microbiology, June 2009, p. 1785-1790, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.01398-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Peptide Nucleic Acid-Based Array for Detecting and Genotyping Human Papillomaviruses{triangledown}

Jae-jin Choi, Chunhee Kim, and Heekyung Park*

Panagene Incorporated, Daejeon 305-510, South Korea

Received 21 July 2008/ Returned for modification 22 September 2008/ Accepted 28 March 2009

We describe a novel array for accurate and reliable genotyping of human papillomavirus (HPV) using peptide nucleic acid (PNA) probes. In order to exploit the superior hybridization properties of PNA with target HPV DNAs, we developed a novel PNA array (PANArray HPV). PANArray HPV enables the detection and genotyping of HPVs using 32 type-specific PNA capture probes for medically important HPVs. All tested HPV types showed highly unique hybridization patterns with type-specific PNA probes. PNA array results showed stable specificities and sensitivities after up to 13 months of storage at room temperature. Also, we demonstrated the superior specificity, sensitivity, and stability of PNA arrays for HPV genotyping. We compared the genotyping results of the PNA array to sequencing with MY09/11 PCR products derived from 72 clinical samples. The results showed excellent agreement between the PNA array and sequencing, except for samples reflecting multiple infections. The results from the PNA array were compared with those of type-specific PCR when discrepant results occurred owing to multiple infections. The results for the PNA array matched those of type-specific PCR in all cases. Newly developed PNA arrays show excellent specificity and sensitivity and long shelf life. Our results suggest that the PNA array represents a reliable alternative to conventional DNA arrays for HPV genotyping, as well as for diagnostics.

* Corresponding author. Mailing address: Panagene Inc., 816 Tamnip-Dong, Youseong-Gu, Daejeon, 305-510, South Korea. Phone: 82-42-861-9295. Fax: 82-42-861-9297. E-mail: hkpark{at}panagene.com

{triangledown} Published ahead of print on 15 April 2009.

Journal of Clinical Microbiology, June 2009, p. 1785-1790, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.01398-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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