This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Taj-Aldeen, S. J.
Right arrow Articles by Boekhout, T.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Taj-Aldeen, S. J.
Right arrow Articles by Boekhout, T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2009, p. 1791-1799, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.02222-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Molecular Identification and Susceptibility of Trichosporon Species Isolated from Clinical Specimens in Qatar: Isolation of Trichosporon dohaense Taj-Aldeen, Meis & Boekhout sp. nov.{triangledown}

Saad J. Taj-Aldeen,1* Nasser Al-Ansari,1 Sittana El Shafei,1 Jacques F. Meis,2 Ilse Curfs-Breuker,2 Bart Theelen,3 and Teun Boekhout3,4

Microbiology Division, Department of Laboratory Medicine and Pathology, Hamad Medical Corporation, P.O. Box 3050, Doha, Qatar,1 Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen,2 CBS Fungal Diversity Centre, Utrecht,,3 Department of Internal Medicine and Infectious Diseases, University Medical Centre, Utrecht, The Netherlands4

Received 19 November 2008/ Returned for modification 13 February 2009/ Accepted 20 March 2009

Trichosporon species have been reported as emerging pathogens and usually occur in severely immunocompromised patients. In the present work, 27 clinical isolates of Trichosporon species were recovered from 27 patients. The patients were not immunocompromised, except for one with acute myeloid leukemia. Sequence analysis revealed the isolation of Trichosporon dohaense Taj-Aldeen, Meis & Boekhout sp. nov., with CBS 10761T as the holotype strain, belonging to the Ovoides clade. In the D1-D2 large-subunit rRNA gene analysis, T. dohaense is a sister species to T. coremiiforme, and in the internal transcribed spacer analysis, the species is basal to the other species of this clade. Molecular identification of the strains yielded 17 T. asahii, 3 T. inkin, 2 T. japonicum, 2 T. faecale, and 3 T. dohaense isolates. The former four species exhibited low MICs for five antifungal azoles but showed high MICs for amphotericin B. T. dohaense demonstrated the lowest amphotericin B MIC (1 mg/liter). For the majority of T. asahii isolates, amphotericin B MICs were high (MIC at which 90% of isolates were inhibited [MIC90], ≥16 mg/liter), and except for fluconazole (MIC90, 8 mg/liter), the azole MICs were low: MIC90s were 0.5 mg/liter for itraconazole, 0.25 mg/liter for voriconazole, 0.25 mg/liter for posaconazole, and 0.125 mg/liter for isavuconazole. The echinocandins, caspofungin and anidulafungin, demonstrated no activity against Trichosporon species.

* Corresponding author. Mailing address: Microbiology Division, Department of Laboratory Medicine and Pathology, Hamad Medical Corporation, P.O. Box 3050, Doha, Qatar. Phone: 974-4392038. Fax: 974-4312751. E-mail: stajaldeen{at}hmc.org.qa

{triangledown} Published ahead of print on 25 March 2009.

Journal of Clinical Microbiology, June 2009, p. 1791-1799, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.02222-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»