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Journal of Clinical Microbiology, June 2009, p. 1885-1887, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.01406-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Enrichment Broth Improved Detection of Extended-Spectrum-Beta-Lactamase-Producing Bacteria in Throat and Rectal Surveillance Cultures of Samples from Patients in Intensive Care Units {triangledown}

Jean-Luc A. N. Murk,1* Edou R. Heddema,2 Dorine L. J. Hess,1 Johannes A. Bogaards,3 Christina M. J. E. Vandenbroucke-Grauls,1 and Yvette J. Debets-Ossenkopp1

VU University Medical Center, Medical Microbiology and Infection Control, Amsterdam, The Netherlands,1 Maaslandhospital, Medical Microbiology, Sittard, The Netherlands,2 VU University Medical Center, Epidemiology and Biostatistics, Amsterdam, The Netherlands3

Received 22 July 2008/ Returned for modification 3 September 2008/ Accepted 9 April 2009

We evaluated the use of a trypticase soy broth (TSB) for improving detection of extended-spectrum-beta-lactamase-producing (ESBL+) bacteria. Preenrichment of throat and rectal swabs in TSB prior to inoculation on solid medium doubled the number of ESBL+ bacteria detected in samples obtained from patients in our intensive care unit.

* Corresponding author. Mailing address: VU University Medical Center, Medical Microbiology and Infection Control, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands. Phone: 31 20 4440488. Fax: 31 20 4440473. E-mail: j.murk{at}vumc.nl

{triangledown} Published ahead of print on 22 April 2009.

Journal of Clinical Microbiology, June 2009, p. 1885-1887, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.01406-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Diederen, B. M. W., Euser, S. M., Murk, J.-L. A. N., Hess, D. L. J., Vandenbroucke-Grauls, C. M. J. E., Debets-Ossenkopp, Y. J., Heddema, E. R., Bogaards, J. A. (2009). Enrichment Broth and Detection of Extended-Spectrum-Beta-Lactamase-Producing Bacteria in Throat and Rectal Surveillance Cultures of Intensive Care Unit Patients. J. Clin. Microbiol. 47: 3066-3067 [Full Text]  


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