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Journal of Clinical Microbiology, June 2009, p. 1892-1895, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.00105-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Development of Loop-Mediated Isothermal Amplification Assay for Detection of Entamoeba histolytica{triangledown}

Shih-Yu Liang,1 Yun-Hsien Chan,2 Kan-Tai Hsia,1 Jing-Lun Lee,2 Ming-Chu Kuo,2 Kuo-Yuan Hwa,3 Chi-Wen Chan,2 Ting-Yi Chiang,2 Jung-Sheng Chen,2 Fang-Tzy Wu,2 and Dar-Der Ji2*

Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei, Taiwan,1 Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan,2 Department of Molecular Science and Engineering, National Taipei University of Technology, Taipei, Taiwan3

Received 18 January 2009/ Returned for modification 27 February 2009/ Accepted 18 March 2009

A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba species, bacteria, and viruses. These results indicate that LAMP is a rapid, simple, and valuable diagnostic tool for epidemiological studies of amebiasis.

* Corresponding author. Mailing address: Research and Diagnostic Center, Centers for Disease Control, No. 161 Kun-Yang Street, Taipei, Taiwan. Phone: 886 2 2653 1367. Fax: 886 2 2783 0877. E-mail: jidarder{at}cdc.gov.tw

{triangledown} Published ahead of print on 25 March 2009.

Journal of Clinical Microbiology, June 2009, p. 1892-1895, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.00105-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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