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Journal of Clinical Microbiology, June 2009, p. 1906-1910, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.00629-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Development and Characterization of a Genotype 4 Hepatitis E Virus Cell Culture System Using a HE-JF5/15F Strain Recovered from a Fulminant Hepatitis Patient {triangledown}

Toshinori Tanaka,1 Masaharu Takahashi,1 Hideyuki Takahashi,1 Koji Ichiyama,1 Yu Hoshino,1 Shigeo Nagashima,1 Hitoshi Mizuo,2 and Hiroaki Okamoto1*

Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, Tochigi-Ken, Japan,1 Department of Internal Medicine, Kin-ikyo Chuo Hospital, Sapporo, Hokkaido, Japan2

Received 28 March 2009/ Accepted 3 April 2009

We developed an efficient cell culture system for genotype 4 hepatitis E virus using the HE-JF5/15F strain recovered from a fulminant hepatitis patient. The sixth-passage virus in the culture supernatant reached 1.5 x 108 copies/ml at 10 days postinoculation and possessed 10 nucleotide mutations with four amino acid changes.

* Corresponding author. Mailing address: Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke-Shi, Tochigi-Ken 329-0498, Japan. Phone: 81-285-58-7404. Fax: 81-285-44-1557. E-mail: hokamoto{at}jichi.ac.jp

{triangledown} Published ahead of print on 15 April 2009.

Journal of Clinical Microbiology, June 2009, p. 1906-1910, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.00629-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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