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Journal of Clinical Microbiology, June 2009, p. 1915-1917, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.00608-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Validation of Five-Colony Pool Analysis Using Multiplex Real-Time PCR for Detection of Diarrheagenic Escherichia coli{triangledown}

F. Barletta,1* T. J. Ochoa,1,2 L. Ecker,3 A. I. Gil,3 C. F. Lanata,3 and T. G. Cleary2

Instituto de Medicina Tropical, Universidad Peruana Cayetano Heredia, Lima, Peru,1 Center for Infectious Diseases, University of Texas School of Public Health, Houston, Texas,2 Instituto de Investigación Nutricional, Lima, Peru3

Received 25 March 2009/ Accepted 27 March 2009

Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis.

* Corresponding author. Mailing address: Instituto de Medicina Tropical, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 340, San Martin de Porras, Lima 33, Perú. Phone: 51-1-482-3910. Fax: 51-1-482-3404. E-mail: francescabarletta{at}yahoo.es

{triangledown} Published ahead of print on 8 April 2009.

Journal of Clinical Microbiology, June 2009, p. 1915-1917, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.00608-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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