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Journal of Clinical Microbiology, June 2009, p. 1931-1933, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.02245-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Outbreak of Hepatitis E Virus Infection in Darfur, Sudan: Effectiveness of Real-Time Reverse Transcription-PCR Analysis of Dried Blood Spots {triangledown}

Audrey Mérens,1* Philippe Jean Guérin,2 Jean-Paul Guthmann,2 and Elisabeth Nicand1

National Reference Laboratory for Hepatitis E, Hospital Val-de-Grâce, Paris, France,1 Epicentre, Paris, France2

Received 21 November 2008/ Returned for modification 3 February 2009/ Accepted 23 March 2009

Biological samples collected in refugee camps during an outbreak of hepatitis E were used to compare the accuracy of hepatitis E virus RNA amplification by real-time reverse transcription-PCR (RT-PCR) for sera and dried blood spots (concordance of 90.6%). Biological profiles (RT-PCR and serology) of asymptomatic individuals were also analyzed.

* Corresponding author. Mailing address: Hôpital d'Instruction des Armées Bégin, 69 Avenue de Paris, 94 160 Saint-Mandé Cedex, France. Phone: 00 (33) 1-40-51-46-30. Fax: 00 (33) 1-43-98-53-36. E-mail: merens-a{at}wanadoo.fr

{triangledown} Published ahead of print on 1 April 2009.

Journal of Clinical Microbiology, June 2009, p. 1931-1933, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.02245-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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