This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kilpatrick, D. R.
Right arrow Articles by Kew, O. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kilpatrick, D. R.
Right arrow Articles by Kew, O. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2009, p. 1939-1941, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.00702-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Rapid Group-, Serotype-, and Vaccine Strain-Specific Identification of Poliovirus Isolates by Real-Time Reverse Transcription-PCR Using Degenerate Primers and Probes Containing Deoxyinosine Residues{triangledown} ,{ddagger}

David R. Kilpatrick,* Chen-Fu Yang,{dagger} Karen Ching, Annelet Vincent, Jane Iber, Ray Campagnoli, Mark Mandelbaum, Lina De, Su-Ju Yang, Allan Nix, and Olen M. Kew

Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333

Received 6 April 2009/ Accepted 9 April 2009

We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.

* Corresponding author. Mailing address: Polio and Picornavirus Laboratory Branch, G-10, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333. Phone: (404) 639-1345. Fax: (404) 639-4011. E-mail: DKilpatrick{at}cdc.gov

{triangledown} Published ahead of print on 22 April 2009.

{ddagger} Supplemental material for this article may be found at http://jcm.asm.org/.

{dagger} Present address: Center for Research and Diagnostics, Centers for Disease Control, Taipei 115, Taiwan.

Journal of Clinical Microbiology, June 2009, p. 1939-1941, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.00702-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»