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Journal of Clinical Microbiology, June 2009, p. 1939-1941, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.00702-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Rapid Group-, Serotype-, and Vaccine Strain-Specific Identification of Poliovirus Isolates by Real-Time Reverse Transcription-PCR Using Degenerate Primers and Probes Containing Deoxyinosine Residues{triangledown} ,{ddagger}

David R. Kilpatrick,* Chen-Fu Yang,{dagger} Karen Ching, Annelet Vincent, Jane Iber, Ray Campagnoli, Mark Mandelbaum, Lina De, Su-Ju Yang, Allan Nix, and Olen M. Kew

Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333

Received 6 April 2009/ Accepted 9 April 2009

We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.

* Corresponding author. Mailing address: Polio and Picornavirus Laboratory Branch, G-10, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333. Phone: (404) 639-1345. Fax: (404) 639-4011. E-mail: DKilpatrick{at}cdc.gov

{triangledown} Published ahead of print on 22 April 2009.

{ddagger} Supplemental material for this article may be found at http://jcm.asm.org/.

{dagger} Present address: Center for Research and Diagnostics, Centers for Disease Control, Taipei 115, Taiwan.

Journal of Clinical Microbiology, June 2009, p. 1939-1941, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.00702-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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