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Journal of Clinical Microbiology, July 2009, p. 2090-2096, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.02048-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Putative Adhesins of Enteropathogenic and Enterohemorrhagic Escherichia coli of Serogroup O26 Isolated from Humans and Cattle {triangledown}

Marjorie Bardiau,* Sabrina Labrozzo, and Jacques G. Mainil

Department of Infectious and Parasitic Diseases—Bacteriology, Veterinary Faculty, University of Liège, Liège B4000, Belgium

Received 16 October 2008/ Returned for modification 25 November 2008/ Accepted 23 April 2009

Enterohemorrhagic Escherichia coli (EHEC) strains are responsible for food poisoning in developed countries via consumption of vegetal and animal food sources contaminated by ruminant feces, and some strains (O26, O111, and O118 serogroups) are also responsible for diarrhea in young calves. The prevalence of 27 putative adhesins of EHEC and of bovine necrotoxigenic E. coli (NTEC) was studied with a collection of 43 bovine and 29 human enteropathogenic (EPEC) and EHEC strains and 5 non-EPEC/non-EHEC (1 bovine and 4 human) O26 strains, using specific PCRs. Four "groups" of adhesins exist, including adhesins present in all O26 strains, adhesins present in most O26 strains, adhesins present in a few O26 strains, and adhesins not present in O26 strains. The common profile of EHEC/EPEC strains was characterized by the presence of loc3, loc5, loc7, loc11, loc14, paa, efa1, iha, lpfAO26, and lpfAO113 genes and the absence of loc1, loc2, loc6, loc12, loc13, saa, and eibG genes. Except for the lpfAO26 gene, which was marginally associated with bovine EHEC/EPEC strains in comparison with human strains (P = 0.012), none of the results significantly differentiated bovine strains from human strains. One adhesin gene (ldaE) was statistically (P < 0.01) associated with O26 EHEC/EPEC strains isolated from diarrheic calves in comparison with strains isolated from healthy calves. ldaE-positive strains could therefore represent a subgroup possessing the specific property of producing diarrhea in young calves. This is the first time that the distribution of putative adhesins has been described for such a large collection of EHEC/EPEC O26 strains isolated from both humans and cattle.

* Corresponding author. Mailing address: Department of Infectious and Parasitic Diseases—Bacteriology, Veterinary Faculty, University of Liège, Liège B4000, Belgium. Phone: 32 4 366 40 52. Fax: 32 4 366 42 63. E-mail: mbardiau{at}ulg.ac.be

{triangledown} Published ahead of print on 29 April 2009.

Journal of Clinical Microbiology, July 2009, p. 2090-2096, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.02048-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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