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Journal of Clinical Microbiology, July 2009, p. 2187-2193, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00304-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Development of a Disk Diffusion Method for Testing Moraxella catarrhalis Susceptibility Using Clinical and Laboratory Standards Institute Methods: a SENTRY Antimicrobial Surveillance Program Report{triangledown}

Jan M. Bell,1 John D. Turnidge,1,2* and Ronald N. Jones3

Antimicrobial Research Laboratory, SA Pathology at the Women's and Children's Hospital, North Adelaide,1 University of Adelaide, Adelaide, South Australia 5006,2 JMI Laboratories, North Liberty, Iowa 523173

Received 10 February 2009/ Returned for modification 14 April 2009/ Accepted 14 May 2009

Currently, there is no Clinical and Laboratory Standards Institute (CLSI) disk diffusion method for testing Moraxella catarrhalis susceptibility. We examined 318 clinical strains of M. catarrhalis obtained as part of the SENTRY (Asia-Pacific) Antimicrobial Surveillance Program, plus two ATCC strains. MICs were determined by the CLSI standard broth microdilution method, and zone diameters were determined on Mueller-Hinton agar incubated in 5% CO2. All strains were examined for the presence of BRO-1 and BRO-2 β-lactamases by using molecular techniques. Tentative zone diameter interpretive criteria were successfully developed for 19 antimicrobial agents, including nine β-lactams, using current MIC interpretive criteria where available or wild-type cutoff values where no prior criteria were available. The proposed interpretive criteria were highly accurate, with ≤0.7% very major (falsely susceptible) and ≤1.0% major (falsely resistant) errors, respectively.

* Corresponding author. Mailing address: SA Pathology at the Women's and Children's Hospital, 72 King William Rd., North Adelaide, South Australia 5006. Phone: 61 8 8161 6873. Fax: 61 8 8161 6189. E-mail: john.turnidge{at}health.sa.gov.au

{triangledown} Published ahead of print on 20 May 2009.

Journal of Clinical Microbiology, July 2009, p. 2187-2193, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00304-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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