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Journal of Clinical Microbiology, July 2009, p. 2252-2255, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00033-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Rapid Identification of Biothreat and Other Clinically Relevant Bacterial Species by Use of Universal PCR Coupled with High-Resolution Melting Analysis{triangledown}

Samuel Yang,1*,{dagger} Padmini Ramachandran,1,{dagger} Richard Rothman,1,2 Yu-Hsiang Hsieh,1 Andrew Hardick,2 Helen Won,2 Aleksandar Kecojevic,1 Joany Jackman,3 and Charlotte Gaydos1,2

Johns Hopkins University, Department of Emergency Medicine, Baltimore, Maryland,1 Johns Hopkins University, Division of Infectious Diseases, Baltimore, Maryland,2 Applied Physics Laboratory, Johns Hopkins University, Laurel, Maryland3

Received 7 January 2009/ Returned for modification 13 February 2009/ Accepted 6 May 2009

A rapid assay for eubacterial species identification is described using high-resolution melt analysis to characterize PCR products. Unique melt profiles generated from multiple hypervariable regions of the 16S rRNA gene for 100 clinically relevant bacterial pathogens, including category A and B biothreat agents and their surrogates, allowed highly specific species identification.

* Corresponding author. Mailing address: Johns Hopkins University Department of Emergency Medicine, 5801 Smith Avenue, Suite 3220, Davis Building, Baltimore, MD 21209. Phone: (410) 735-6441. Fax: (410) 735-6440. E-mail: syang10{at}jhmi.edu

{triangledown} Published ahead of print on 20 May 2009.

{dagger} Samuel Yang and Padmini Ramachandran both contributed equally to the manuscript.

Journal of Clinical Microbiology, July 2009, p. 2252-2255, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00033-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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