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Journal of Clinical Microbiology, July 2009, p. 2260-2264, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00544-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Single Nucleotide Polymorphisms in the IS900 Sequence of Mycobacterium avium subsp. paratuberculosis Are Strain Type Specific{triangledown}

Elena Castellanos,1,2 Alicia Aranaz,1,2* Lucia de Juan,1,2 Julio Álvarez,1 Sabrina Rodríguez,1,2 Beatriz Romero,1,2 Javier Bezos,1,2 Karen Stevenson,3 Ana Mateos,1,2 and Lucas Domínguez1,2

Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain,1 Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain,2 Moredun Research Institute, Division of Control of Bacterial Diseases, Pentlands Science Park, Bush Loan, Penicuik, EH26 OPZ Scotland, United Kingdom3

Received 17 March 2009/ Accepted 27 April 2009

Insertion sequence IS900 is used as a target for the identification of Mycobacterium avium subsp. paratuberculosis. Previous reports have revealed single nucleotide polymorphisms within IS900. This study, which analyzed the IS900 sequences of a panel of isolates representing M. avium subsp. paratuberculosis strain types I, II, and III, revealed conserved type-specific polymorphisms that could be utilized as a tool for diagnostic and epidemiological purposes.

* Corresponding author. Mailing address: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain. Phone: (34) 913943721. Fax: (34) 913943795. E-mail: alaranaz{at}vet.ucm.es

{triangledown} Published ahead of print on 13 May 2009.

Journal of Clinical Microbiology, July 2009, p. 2260-2264, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00544-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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