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Journal of Clinical Microbiology, July 2009, p. 2269-2271, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00326-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Evaluation of Five Commercial Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in Respiratory Tract Specimens {triangledown}

A. Touati,1 A. Benard,2 A. Ben Hassen,1 C. M. Bébéar,3* and S. Pereyre3

Service des Laboratoires, Centre National de Greffe de Moelle Osseuse, Tunis, Tunisia,1 Unité de Soutien Méthodologique à la Recherche Clinique et Epidémiologique, INSERM U897, CIC-EC 7, Université Victor Segalen Bordeaux 2 and CHU de Bordeaux, Bordeaux, France,2 Laboratoire de Bactériologie EA 3671, Université Victor Segalen Bordeaux 2 and CHU de Bordeaux, Bordeaux, France3

Received 13 February 2009/ Returned for modification 18 April 2009/ Accepted 21 April 2009

The performances of five commercial TaqMan real-time PCR assays for the detection of Mycoplasma pneumoniae in respiratory tract specimens were evaluated in comparison with an in-house real-time PCR. All kits allowed prompt and specific results, validated by the use of an internal control. The Nanogen kit showed the best clinical sensitivity.

* Corresponding author. Mailing address: Laboratoire de Bactériologie EA 3671, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, France. Phone: (33) 5 57 57 16 25. Fax: (33) 5 56 93 29 40. E-mail: cecile.bebear{at}u-bordeaux2.fr

{triangledown} Published ahead of print on 29 April 2009.

Journal of Clinical Microbiology, July 2009, p. 2269-2271, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00326-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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