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Journal of Clinical Microbiology, July 2009, p. 2301-2303, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.02366-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Use of Reverse Transcriptase PCR To Subtype N1 to N9 Neuraminidase Genes of Avian Influenza Viruses{triangledown}

Kenji Tsukamoto,1* Takayoshi Ashizawa,2 Koji Nakanishi,3 Noriyuki Kaji,4 Kotaro Suzuki,1 Makiko Shishido,1 Masatoshi Okamatsu,1 and Masaji Mase1

Research Team for Zoonotic Diseases, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan,1 Tyuou Livestock Hygiene Service Center of Chiba Prefecture, Iwatomi, Sakura, Chiba 285-0072, Japan,2 Livestock Hygiene Service Center of Shiga Prefecture, Nishihongo, Oomi-hachiman, Shiga 523-0813, Japan,3 Livestock Hygiene Service Center of Shimane Prefecture, Jinzaioki, Izumo, Shimane 699-0822, Japan4

Received 9 December 2008/ Returned for modification 13 March 2009/ Accepted 4 April 2009

Reverse transcriptase PCR designed to amplify the N1 to N9 neuraminidase (NA) genes of avian influenza viruses detected 118 of the 119 NA genes tested (99.2%) in a subtype-specific manner. This technique successfully subtyped all 167 recent avian influenza viruses isolated from birds. Subtype specificity was confirmed by sequence analyses of all 285 PCR products.

* Corresponding author. Mailing address: Research Team for Zoonotic Diseases, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan. Phone: 81-29-838-7802. Fax: 81-29-838-7802. E-mail: ktsukamo{at}affrc.go.jp

{triangledown} Published ahead of print on 29 April 2009.

Journal of Clinical Microbiology, July 2009, p. 2301-2303, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.02366-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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