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Journal of Clinical Microbiology, July 2009, p. 2304-2306, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00498-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus{triangledown}

Xi Lan, Bin Yang, Bao Yu Li, Xiang Ping Yin, Xue Rui Li, and Ji Xing Liu*

Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, People's Republic of China

Received 10 March 2009/ Returned for modification 1 April 2009/ Accepted 24 April 2009

The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63°C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a detection limit of 0.045 fg (nine copies/reaction).

* Corresponding author. Mailing address: Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, People's Republic of China. Phone: 86-931-8342682. Fax: 86-931-8340977. E-mail: liujixing{at}hotmail.com

{triangledown} Published ahead of print on 29 April 2009.

Journal of Clinical Microbiology, July 2009, p. 2304-2306, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00498-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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