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Journal of Clinical Microbiology, July 2009, p. 2307-2310, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00240-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Pyrosequencing Analysis of 20 Nucleotides of Internal Transcribed Spacer 2 Discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis{triangledown}

Andrew M. Borman,1* Christopher J. Linton,1 Debra Oliver,1 Michael D. Palmer,1 Adrien Szekely,1 Frank C. Odds,2 and Elizabeth M. Johnson1

Mycology Reference Laboratory, Health Protection Agency, Bristol, United Kingdom,1 Aberdeen Fungal Group, School of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom2

Received 4 February 2009/ Returned for modification 9 April 2009/ Accepted 21 April 2009

Two new cryptic sister species, Candida orthopsilosis and Candida metapsilosis, were recently identified by consistent DNA sequence differences among several genes within the genetically heterogeneous Candida parapsilosis complex. Here, we present data demonstrating that Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between these three closely related Candida species.

* Corresponding author. Mailing address: Mycology Reference Laboratory, Health Protection Agency South-West Regional Laboratory, Myrtle Road, Kingsdown, Bristol BS2 8EL, United Kingdom. Phone: 0117 926 8683. Fax: 0117 922 6611. E-mail: Andy.Borman{at}uhBristol.nhs.uk

{triangledown} Published ahead of print on 29 April 2009.

Journal of Clinical Microbiology, July 2009, p. 2307-2310, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00240-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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