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Journal of Clinical Microbiology, August 2009, p. 2377-2380, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.02512-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of Atypical Isolates of Yersinia intermedia and Definition of Two New Biotypes{triangledown} ,{dagger}

Liliane Martin, Alexandre Leclercq,{ddagger} Cyril Savin, and Elisabeth Carniel*

Institut Pasteur, Yersinia Research Unit, Yersinia National Reference Laboratory, Paris, France

Received 31 December 2008/ Accepted 20 May 2009

The species Yersinia intermedia is a member of the genus Yersinia which belongs to the Enterobacteriaceae family. This species is divided into eight biotypes, according to Brenner's biotyping scheme. This scheme relies on five tests (utilization of Simmons citrate and acid production from D-melibiose, D-raffinose, {alpha}-methyl-D-glucoside [{alpha}MG], and L-rhamnose). The collection of the French Yersinia Reference Laboratory (Institut Pasteur, Paris, France) contained 44 strains that were originally identified as Y. intermedia but whose characteristics did not fit into the biotyping scheme. These 44 strains were separated into two biochemical groups: variant 1 (positive for acid production from L-rhamnose and {alpha}MG and positive for Simmons citrate utlization) and variant 2 (positive for acid production from L-rhamnose and {alpha}MG). These atypical strains could correspond to new biotypes of Y. intermedia, to Y. frederiksenii strains having the atypical property of fermenting {alpha}MG, or to new Yersinia species. These strains did not exhibit growth or phenotypic properties different from those of Y. intermedia and Y. frederiksenii and did not harbor any of the virulence traits usually found in pathogenic species. DNA-DNA hybridizations performed between one strain each of variants 1 and 2 and the Y. intermedia and Y. frederiksenii type strains demonstrated that these variants do belong to the Y. intermedia species. We thus propose that Brenner's biotyping scheme be updated by adding two new biotypes: 9 (for variant 1) and 10 (for variant 2) to the species Y. intermedia.

* Corresponding author. Mailing address: Institut Pasteur, Yersinia Research Unit, 28 rue du Dr Roux, Paris 75724 cedex 15, France. Phone: 33 1 40 61 83 26. Fax: 33 1 40 61 30 01. E-mail: elisabeth.carniel{at}pasteur.fr

{triangledown} Published ahead of print on 3 June 2009.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

{ddagger} Present address: Institut Pasteur, CNR Listeria, 25-28 rue du Docteur Roux, Paris 75724 cedex 15, France.

Journal of Clinical Microbiology, August 2009, p. 2377-2380, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.02512-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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