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Journal of Clinical Microbiology, August 2009, p. 2392-2397, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.02379-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Prevalence, Distribution, and Antifungal Susceptibility Profiles of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis in a Tertiary Care Hospital{triangledown}

Ana P. Silva,1* Isabel M. Miranda,1,2 Carmen Lisboa,1 Cidália Pina-Vaz,1,2,3 and Acácio G. Rodrigues1,2,4

Department of Microbiology, Faculty of Medicine, University of Porto,1 Cardiovascular Research & Development Unit, Faculty of Medicine, University of Porto,2 Department of Microbiology, Hospital S. João,3 Burn Unit and Department of Plastic and Reconstructive Surgery, Hospital S. João, Porto, Portugal4

Received 11 December 2008/ Returned for modification 25 January 2009/ Accepted 26 May 2009

Candida parapsilosis, an emergent agent of nosocomial infections, was previously made up of a complex of three genetically distinct groups (groups I, II, and III). Recently, the C. parapsilosis groups have been renamed as distinct species: C. parapsilosis sensu stricto, C. orthopsilosis, and C. metapsilosis. In Portugal, no data pertaining to the distribution and antifungal susceptibility of these Candida species are yet available. In the present report, we describe the incidence and distribution of C. parapsilosis sensu stricto, C. orthopsilosis, and C. metapsilosis among 175 clinical and environmental isolates previously identified by conventional methods as C. parapsilosis. We also evaluated the in vitro susceptibilities of the isolates to fluconazole, voriconazole, posaconazole, amphotericin B, and two echinocandins, caspofungin and anidulafungin. Of the 175 isolates tested, 160 (91.4%) were identified as C. parapsilosis sensu stricto, 4 (2.3%) were identified as C. orthopsilosis, and 5 (2.9%) were identified as C. metapsilosis. Six isolates corresponded to species other than the C. parapsilosis group. Interestingly, all isolates from blood cultures corresponded to C. parapsilosis sensu stricto. Evaluation of the antifungal susceptibility profile showed that only nine (5.6%) C. parapsilosis sensu stricto strains were susceptible-dose dependent or resistant to fluconazole, and a single strain displayed a multiazole-resistant phenotype; two (1.3%) C. parapsilosis sensu stricto strains were amphotericin B resistant. All C. orthopsilosis and C. metapsilosis isolates were susceptible to azoles and amphotericin B. A high number of strains were nonsusceptible to the echinocandins (caspofungin and anidulafungin).

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Medicine, University of Porto, Alameda Hernâni Monteiro, Porto 4200-319, Portugal. Phone and fax: 00351225513662. E-mail: anatpsilva{at}hotmail.com

{triangledown} Published ahead of print on 3 June 2009.

Journal of Clinical Microbiology, August 2009, p. 2392-2397, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.02379-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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