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Journal of Clinical Microbiology, August 2009, p. 2470-2474, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00484-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Simple, Accurate, Serotype-Specific PCR Assay To Differentiate Streptococcus pneumoniae Serotypes 6A, 6B, and 6C{triangledown} ,{dagger}

Ping Jin,1,2,{ddagger} Meng Xiao,3,{ddagger} Fanrong Kong,2,{ddagger} Shahin Oftadeh,2 Fei Zhou,2 Chunyi Liu,1 and Gwendolyn L. Gilbert2*

Bao'an Maternity and Child Health Hospital, Shenzhen, People's Republic of China,1 Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead, New South Wales, Australia,2 Life Science College, Peking University, Beijing, People's Republic of China3

Received 9 March 2009/ Returned for modification 15 May 2009/ Accepted 8 June 2009

In this study, we developed a simple, reliable, serotype-specific PCR method to differentiate Streptococcus pneumoniae serotypes 6A, 6B, and 6C. It was more efficient and practical than the assays currently being used to identify serotypes 6A, 6B, and 6C. Of 120 selected serogroup 6 isolates from subjects with invasive (n = 101) and noninvasive (n = 19) pneumococcal disease, most of which were collected after 2003 in New South Wales, 45 had been identified as 6A and 75 had been identified as 6B by the Quellung reaction. PCR analysis confirmed the results for serotype 6B isolates and identified two different subtypes. Fourteen of 45 isolates that had been identified as serotype 6A actually belonged to serotype 6C.

* Corresponding author. Centre for Infectious Diseases and Microbiology (CIDM), Institute of Clinical Pathology and Medical Research (ICPMR), Westmead Hospital, Darcy Road, Westmead, New South Wales, 2145 Australia. Phone: (612) 9845 6255. Fax: (612) 9893 8659. E-mail: l.gilbert{at}usyd.edu.au

{triangledown} Published ahead of print on 17 June 2009.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

{ddagger} These authors contributed equally to the work.

Journal of Clinical Microbiology, August 2009, p. 2470-2474, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00484-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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