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Journal of Clinical Microbiology, August 2009, p. 2483-2488, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.02182-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Proteomic Identification of OprL as a Seromarker for Initial Diagnosis of Pseudomonas aeruginosa Infection of Patients with Cystic Fibrosis{triangledown}

Aparna R. Rao,1 Anita Laxova,2 Philip M. Farrell,2 and Joseph T. Barbieri3*

Department of Pediatrics,1 Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin,3 Department of Pediatrics, University of Wisconsin Medical School, Madison, Wisconsin2

Received 13 November 2008/ Returned for modification 13 February 2009/ Accepted 18 June 2009

Identification of new immunogenic antigens that diagnose initial Pseudomonas aeruginosa infections in patients with cystic fibrosis (CF) alone or as an adjunct to microbiology is needed. In the present study, a proteomic analysis was performed to obtain a global assessment of the host immune response during the initial P. aeruginosa infection of patients with CF. Matrix-assisted laser desorption ionization-time of flight mass spectrometry was used to identify outer membrane protein L (OprL), a non-type III secretion system (TTSS) protein, as an early immunogenic protein during the initial P. aeruginosa infection of patients with CF. Longitudinal Western blot analysis of sera from 12 of 14 patients with CF detected antibodies to OprL during the initial P. aeruginosa infection. In addition, also detected were antibodies to ExoS, ExoU, or ExoS and ExoU, the latter indicating sequential P. aeruginosa infections during initial infections. Detection of serum reactivity to OprL, along with proteins of the TTSS, and in conjunction with microbiology may diagnose initial P. aeruginosa infections in patients with CF.

* Corresponding author. Mailing address: Department of Microbiology, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 5322-0509. Phone: (414) 456-8412. Fax: (414) 456-6535. E-mail: jtb01{at}mcw.edu

{triangledown} Published ahead of print on 24 June 2009.

Journal of Clinical Microbiology, August 2009, p. 2483-2488, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.02182-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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