This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Sun, S.
Right arrow Articles by Zhang, Y.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sun, S.
Right arrow Articles by Zhang, Y.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2009, p. 2536-2543, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00265-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Epidemiology and Phylogenetic Analysis of Crimean-Congo Hemorrhagic Fever Viruses in Xinjiang, China{triangledown}

Surong Sun,1 Xiang Dai,2 Muhetaer Aishan,2 Xinhui Wang,2 Weiwei Meng,2 Conghui Feng,2 Fuchun Zhang,1 Changshou Hang,3 Zhihong Hu,4* and Yujiang Zhang2*

Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urmuqi, China,1 The Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi, China,2 Institute of Virology, Chinese Center for Disease Control and Prevention, Beijing, China,3 State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China4

Received 6 February 2009/ Returned for modification 25 May 2009/ Accepted 7 June 2009

In 2004 and 2005, an epidemiological survey of Crimean-Congo hemorrhagic fever virus (CCHFV) was conducted in Xinjiang, China. A total of 5,629 serum samples of human and livestock were collected and tested for the CCHFV antibody, and 17,319 ticks were collected for viral identification. Reverse passive hemagglutination inhibition assays showed that the average prevalence of CCHFV antibody was 1.7% for the humans and 12.7% for the livestock. A relatively high antibody prevalence, ranging from 19.1% to 23.4%, was found in the livestock of the northwest, southwest, and northeast parts of the Tarim Basin. When the ticks were pooled to inoculate suckling mice, followed by reverse transcription-PCR (RT-PCR) to detect CCHFV RNA, the average RT-PCR-positive rates for Hyalomma asiaticum kozlovi and H. asiaticum asiaticum were 12.9% and 2.6%, respectively. A significant correlation was found between the antibody prevalence in the livestock and the CCHFV prevalence in H. asiaticum of the same geographic region. No CCHFV RNA was detected in Dermacentor nivenus, Rhipicephalus turanius, or Rhipicephalus sanguineus. A total of 27 partial S segments of CCHFVs were sequenced and used for phylogeny analysis. All but one Chinese isolate grouped into the Asia 1 clade, which contains the strains from Xinjiang and Uzbekistan, while the other strain, Fub90009, grouped with strains from the Middle East.

* Corresponding author. Mailing address for Zhihong Hu: State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan 430071, China. Phone and fax: 86-27-87197180. E-mail: huzh{at}wh.iov.cn. Mailing address for Yujiang Zhang: The Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, 4 Jianquan First Street, Urumuqi, Xinjiang 830046, China. Phone: 86-991-2650698. Fax: 86-991-2650698. E-mail: xjsyzhang{at}163.com

{triangledown} Published ahead of print on 24 June 2009.

Journal of Clinical Microbiology, August 2009, p. 2536-2543, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00265-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»