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Journal of Clinical Microbiology, August 2009, p. 2564-2570, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00285-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Detection and Genotyping of Human Papillomavirus in Self-Obtained Cervicovaginal Samples by Using the FTA Cartridge: New Possibilities for Cervical Cancer Screening {triangledown}

Charlotte H. Lenselink,1 Roosmarie P. de Bie,1 Dennis van Hamont,1 Judith M. J. E. Bakkers,2 Wim G. V. Quint,3 Leon F. A. G. Massuger,1 Ruud L. M. Bekkers,1 and Willem J. G. Melchers2*

Department of Obstetrics and Gynecology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands,1 Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands,2 DDL Diagnostic Laboratory, Voorburg, The Netherlands3

Received 9 February 2009/ Returned for modification 24 April 2009/ Accepted 17 June 2009

This study assesses human papillomavirus (HPV) detection and genotyping in self-sampled genital smears applied to an indicating FTA elute cartridge (FTA cartridge). The study group consisted of 96 women, divided into two sample sets. All samples were analyzed by the HPV SPF10-Line Blot 25. Set 1 consisted of 45 women attending the gynecologist; all obtained a self-sampled cervicovaginal smear, which was applied to an FTA cartridge. HPV results were compared to a cervical smear (liquid based) taken by a trained physician. Set 2 consisted of 51 women who obtained a self-sampled cervicovaginal smear at home, which was applied to an FTA cartridge and to a liquid-based medium. DNA was obtained from the FTA cartridges by simple elution as well as extraction. Of all self-obtained samples of set 1, 62.2% tested HPV positive. The overall agreement between self- and physician-obtained samples was 93.3%, in favor of the self-obtained samples. In sample set 2, 25.5% tested HPV positive. The overall agreement for high-risk HPV presence between the FTA cartridge and liquid-based medium and between DNA elution and extraction was 100%. This study shows that HPV detection and genotyping in self-obtained cervicovaginal samples applied to an FTA cartridge is highly reliable. It shows a high level of overall agreement with HPV detection and genotyping in physician-obtained cervical smears and liquid-based self-samples. DNA can be obtained by simple elution and is therefore easy, cheap, and fast. Furthermore, the FTA cartridge is a convenient medium for collection and safe transport at ambient temperatures. Therefore, this method may contribute to a new way of cervical cancer screening.

* Corresponding author. Mailing address: Radboud University Nijmegen Medical Centre, Department of Medical Microbiology (Internal Mail 574), P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. Phone: 31 (0)243614384. Fax: 31 (0)243540216. E-mail: W.Melchers{at}mmb.umcn.nl

{triangledown} Published ahead of print on 24 June 2009.

Journal of Clinical Microbiology, August 2009, p. 2564-2570, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00285-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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