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Journal of Clinical Microbiology, August 2009, p. 2586-2589, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00997-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Development of a Multiplex Real-Time PCR for Detection and Differentiation of Malignant Catarrhal Fever Viruses in Clinical Samples {triangledown}

Cristina W. Cunha,1* Lisa Otto,2 Naomi S. Taus,1 Donald P. Knowles,1,2 and Hong Li1

Animal Disease Research Unit, USDA-ARS, Washington State University, P.O. Box 646630, Pullman, Washington 99164-6630,1 College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-70402

Received 19 May 2009/ Accepted 22 May 2009

A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%).

* Corresponding author. Mailing address: Animal Disease Research Unit, ARS, USDA, 3003 ADBF, Washington State University, Pullman, WA 99164-6630. Phone: (509) 335-6335. Fax: (509) 335-8328. E-mail: cwcunha{at}vetmed.wsu.edu

{triangledown} Published ahead of print on 3 June 2009.

Journal of Clinical Microbiology, August 2009, p. 2586-2589, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00997-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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