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Journal of Clinical Microbiology, August 2009, p. 2590-2592, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00013-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Detection of Cryptic Genospecies Misidentified as Haemophilus influenzae in Routine Clinical Samples by Assessment of Marker Genes fucK, hap, and sodC{triangledown}

Niels Nørskov-Lauritsen*

Department of Clinical Microbiology, Aarhus University Hospital Skejby, DK-8200 Aarhus N, Denmark

Received 4 January 2009/ Returned for modification 12 March 2009/ Accepted 7 June 2009

Clinical isolates of Haemophilus influenzae were assessed for the presence of fucK, hap, and sodC by hybridization with gene-specific probes, and isolates diverging from the expected H. influenzae genotype were characterized by phenotype and 16S rRNA gene sequencing. Two of 480 isolates were finally classified as variant strains ("nonhemolytic Haemophilus haemolyticus").


* Mailing address: Department of Clinical Microbiology, Aarhus University Hospital Skejby, 100 Brendstrupgaardsvej, DK-8200 Aarhus N, Denmark. Phone: 45 8949 5603. Fax: 45 8949 5611. E-mail: nielnoer{at}rm.dk

{triangledown} Published ahead of print on 17 June 2009.


Journal of Clinical Microbiology, August 2009, p. 2590-2592, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00013-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.