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Journal of Clinical Microbiology, August 2009, p. 2593-2595, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00367-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Rapid and Simple Determination of Ciprofloxacin Resistance in Clinical Strains of Escherichia coli{triangledown}

Rebeca Santiso,1,2 María Tamayo,1,2 José Luis Fernández,1,2* María del Carmen Fernández,3 Francisca Molina,3 Rosa Villanueva,3 Jaime Gosálvez,4 and Germán Bou3*

INIBIC-Complejo Hospitalario Universitario Juan Canalejo, Unidad de Genética, As Xubias 84, 15006-A Coruña, Spain,1 Laboratorio de Genética Molecular y Radiobiología, Centro Oncológico de Galicia, Avda. de Montserrat s/n, 15009-A Coruña, Spain,2 INIBIC-Complejo Hospitalario Universitario Juan Canalejo, Servicio de Microbiología, As Xubias 84, 15006-A Coruña, Spain,3 Unidad de Genética, Facultad de Biología, Universidad Autónoma de Madrid, 28049 Madrid, Spain4

Received 18 February 2009/ Accepted 19 June 2009

We recently reported a simple new in situ diffusion assay, developed as a kit, to visualize DNA fragmentation in single bacterial cells. Use of this assay in a collection of 95 genetically unrelated Escherichia coli clinical strains resulted in correct identification of all of the isolates as resistant or susceptible to ciprofloxacin, consistent with the MIC results. This relevant information is obtained in 80 min.

* Corresponding author. Mailing address for José Luis Fernández: Sección de Genética y Unidad de Investigación, Complejo Hospitalario Universitario Juan Canalejo, As Xubias 84, 15006-A Coruña, Spain. Phone: 34 981 287499. Fax: 34 981 287122. E-mail: JLFernandez{at}canalejo.org. Mailing address for Germán Bou: Laboratorio de Microbiología, Complejo Hospitalario Universitario Juan Canalejo, Xubias s/n, 3a Planta Ed. Sur, 15006-A Coruña, Spain. Phone: 34 981 176087. Fax: 34 981 176097. E-mail: germanbou{at}canalejo.org

{triangledown} Published ahead of print on 1 July 2009.

Journal of Clinical Microbiology, August 2009, p. 2593-2595, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00367-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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