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Journal of Clinical Microbiology, August 2009, p. 2596-2600, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00037-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Inaccuracy of Single-Target Sequencing for Discriminating Species of the Mycobacterium abscessus Group{triangledown}

Edouard Macheras,1,3 Anne-Laure Roux,2,3 Fabienne Ripoll,3 Valérie Sivadon-Tardy,1,3 Cristina Gutierrez,4 Jean-Louis Gaillard,1,2,3 and Beate Heym1,3*

Laboratoire de Microbiologie, Hôpital Ambroise Paré, AP-HP, Boulogne-Billancourt,1 Laboratoire de Microbiologie, Hôpital Raymond Poincaré, AP-HP, Garches,2 EA 3647, Université de Versailles Saint-Quentin-en-Yvelines, Garches,3 INSERM 629, Institut Pasteur, Lille, France4

Received 8 January 2009/ Returned for modification 2 April 2009/ Accepted 29 May 2009

We determined nucleotide sequences of rpoB, hsp65, and sodA in 59 clinical isolates (from 58 patients) of the Mycobacterium abscessus group. Identification to the species level, based on three target genes, was concordant for 44 isolates (25 M. abscessus, 13 Mycobacterium massiliense, and 6 Mycobacterium bolletii isolates) and discordant for 15 isolates which had "interspecific composite patterns." Sequence analysis of five housekeeping genes also showed composite patterns in 8 of these 15 isolates.

* Corresponding author. Mailing address: Laboratoire de Microbiologie-Hygiène, Hôpital Ambroise Paré, 9 Avenue Charles de Gaulle, 92100 Boulogne-Billancourt, France. Phone: 33 1 49 09 44 21. Fax: 33 1 49 09 59 21. E-mail: beate.heym{at}apr.aphp.fr

{triangledown} Published ahead of print on 10 June 2009.

Journal of Clinical Microbiology, August 2009, p. 2596-2600, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00037-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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