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Journal of Clinical Microbiology, August 2009, p. 2607-2610, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00770-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Expanded Multilocus Sequence Typing for Burkholderia Species{triangledown} ,{dagger}

Theodore Spilker,1 Adam Baldwin,2 Amy Bumford,1 Chris G. Dowson,2 Eshwar Mahenthiralingam,3 and John J. LiPuma1*

Division of Infectious Diseases, Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan,1 Department of Biological Sciences, Warwick University, Coventry, United Kingdom,2 Cardiff School of Biosciences, Cardiff University, Cardiff, United Kingdom3

Received 16 April 2009/ Returned for modification 18 May 2009/ Accepted 24 May 2009

PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.

* Corresponding author. Mailing address: Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, 8323 MSRB III, SPC 5646, 1150 W. Medical Center Drive, Ann Arbor, MI 48109-0646. Phone: (734) 936-9767. Fax: (734) 764-4279. E-mail: jlipuma{at}umich.edu

{triangledown} Published ahead of print on 3 June 2009.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, August 2009, p. 2607-2610, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00770-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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