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Journal of Clinical Microbiology, August 2009, p. 2620-2622, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.02436-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Evaluation of MeningoFinder, a Novel Multiplex Ligation-Dependent Probe Amplification Assay for Simultaneous Detection of Six Virus Species Causing Central Nervous System Infections{triangledown}

P. F. G. Wolffs,1* C. Vink,1,# J. Keijdener,2 B. Habek,3 M. Reijans,2 G. Simons,2 C. A. Bruggeman,1 and A. J. C. van den Brule3

Department of Medical Microbiology, Maastricht University Medical Center, 6202 AZ Maastricht, The Netherlands,1 PathoFinder B.V., 6229 EV Maastricht, The Netherlands,2 Laboratory for Pathology and Medical Microbiology, PAMM Laboratories, 5623 EJ Eindhoven, The Netherlands3

Received 19 December 2008/ Returned for modification 3 February 2009/ Accepted 18 June 2009

A multiplex ligation-dependent probe amplification assay for simultaneous detection of six virus species was developed and tested on clinical cerebrospinal fluid (CSF) samples. The assay, termed MeningoFinder, showed an accordance of 97%, concordance of 96%, interlaboratory sensitivity of 90%, and interlaboratory specificity of 94% compared to PCRs.

* Corresponding author. Mailing address: Department of Medical Microbiology, University Hospital Maastricht, P. O. Box 5800, 6202 AZ Maastricht, The Netherlands. Phone: 31 43 3876669. Fax: 31 43 3876643. E-mail: p.wolffs{at}mumc.nl

{triangledown} Published ahead of print on 24 June 2009.

# Present address: Laboratory of Pediatrics, Erasmus Medical Center Rotterdam, 3000 CA Rotterdam, The Netherlands.

Journal of Clinical Microbiology, August 2009, p. 2620-2622, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.02436-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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