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Journal of Clinical Microbiology, August 2009, p. 2626-2628, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00893-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Recovery of Anti-Toxoplasma gondii Immunoglobulin M in Stored Guthrie Card Blood Spots{triangledown}

Hooi-Kuan Tan,1 Eskild Petersen,2 Lone N. Møller,3,§ Pamela Phillips,1 Eurico Camargo Neto,4 and Ruth E. Gilbert1*

MRC Centre of Epidemiology for Child Health, Institute of Child Health, London, United Kingdom,1 Department of Infectious Diseases, Aarhus University Hospital, Skejby, DK-8200 Aarhus N., Denmark,2 Department of Bacteriology, Mycology & Parasitology, Statens Serum Institut, 5 Artillerivej, DK-2300 Copenhagen S., Denmark,3 Laboratorio Nobel and Centro de Triagem Neonatal, Porto Alegre, Brazil4

Received 6 May 2009/ Accepted 26 May 2009

Retrospective testing of neonatal Guthrie card blood spots for specific immunoglobulin M (IgM) can distinguish congenital toxoplasmosis from acquired toxoplasmosis. We determined whether storage temperature reduced IgM detection, using filter paper blood samples "spiked" with anti-Toxoplasma IgM. After 300 days, IgM detection deteriorated with storage at room temperature but not at temperatures of 4°C or lower.

* Corresponding author. Mailing address: MRC Centre of Epidemiology for Child Health, Institute of Child Health, 30, Guilford Street, London WC1N 1EH, United Kingdom. Phone: 0207 905 2101. Fax: 0207 831 8233. E-mail: r.gilbert{at}ich.ucl.ac.uk

{triangledown} Published ahead of print on 3 June 2009.

§ Present address: PAARISA, Centre of Public Health, Greenland Home Rule Council, P.O. Box 1160, DK-3900 Nuuk, Greenland.

Journal of Clinical Microbiology, August 2009, p. 2626-2628, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00893-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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