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Journal of Clinical Microbiology, August 2009, p. 2629-2631, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00821-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Evaluation of New Preanalysis Sample Treatment Tools and DNA Isolation Protocols To Improve Bacterial Pathogen Detection in Whole Blood{triangledown}

Wendy L. J. Hansen, Cathrien A. Bruggeman, and Petra F. G. Wolffs*

Department of Medical Microbiology, Maastricht University Medical Center, Maastricht, The Netherlands

Received 24 April 2009/ Returned for modification 21 May 2009/ Accepted 5 June 2009

Two novel preanalysis sample treatment tools were evaluated in combination with four DNA extraction kits for the selective isolation of bacterial DNA from whole blood. The combination of performing a preanalysis sample treatment and using a larger sample volume increased the detection limit to 50 CFU per ml.

* Corresponding author. Mailing address: Department of Medical Microbiology, Maastricht University Medical Center, P. Debyelaan 25, Maastricht, 6202 AZ, The Netherlands. Phone: 31-43-3876669. Fax: 31-43-3876643. E-mail: p.wolffs{at}mumc.nl

{triangledown} Published ahead of print on 17 June 2009.

Journal of Clinical Microbiology, August 2009, p. 2629-2631, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00821-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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