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Journal of Clinical Microbiology, August 2009, p. 2635-2638, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.02170-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Detection and Quantification of Hepatitis C Virus (HCV) by MultiCode-RTx Real-Time PCR Targeting the HCV 3' Untranslated Region{triangledown}

Elizabeth K. Mulligan,1 J. J. Germer,2 Max Q. Arens,3 Krista L. D'Amore,4 Adrian Di Bisceglie,5 Nathan A. Ledeboer,4,6 Michael J. Moser,1 Andrew C. Newman,1 Andy K. O'Guin,7 Paul D. Olivo,7 Diane S. Podzorski,6 Kimberly A. Vaughan,6 Joseph D. Yao,2 Slava A. Elagin,1 and Scott C. Johnson1*

EraGen Biosciences, Inc., Madison, Wisconsin,1 Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota,2 Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri,3 Department of Pathology, Medical College of Wisconsin, Milwaukee, Wisconsin,4 Department of Medicine, Division of Gastrointestinal Medicine, St. Louis University School of Medicine, St. Louis, Missouri,5 Dynacare Laboratories, Milwaukee, Wisconsin,6 Apath LLC, St. Louis, Missouri7

Received 12 November 2008/ Returned for modification 22 December 2008/ Accepted 7 June 2009

A prototype, real-time reverse-transcription PCR assay, based on MultiCode-RTx technology, quantifying hepatitis C virus (HCV) RNA by targeting the HCV 3' untranslated region demonstrated linearity over 7 logs, with a good correlation between the quantitative results of this assay and the results of two commercially available comparator assays for 466 clinical specimens comprising all six HCV genotypes.

* Corresponding author. Mailing address: EraGen Biosciences, Inc., 918 Deming Way, Madison, WI 53717. Phone: (608) 662-9000. Fax: (608) 662-9003. E-mail: sjohnson{at}eragen.com

{triangledown} Published ahead of print on 17 June 2009.

Journal of Clinical Microbiology, August 2009, p. 2635-2638, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.02170-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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