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Journal of Clinical Microbiology, August 2009, p. 2647-2650, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00721-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Duplex PCR Assay Simultaneously Detecting and Differentiating Bartonella quintana, B. henselae, and Coxiella burnetii in Surgical Heart Valve Specimens{triangledown}

Yi-Wei Tang*

Departments of Pathology and Medicine, Vanderbilt University Medical Center, Nashville, Tennessee

Received 8 April 2009/ Returned for modification 24 May 2009/ Accepted 16 June 2009

A duplex PCR (dPCR) assay was developed to simultaneously detect and differentiate Bartonella quintana, Bartonella henselae, and Coxiella burnetii from surgical heart valve tissue specimens with an analytic sensitivity of 10 copies/reaction. Among 17 specimens collected from patients with a clinical diagnosis of culture-negative endocarditis, 2, 4, and 2 were positive for B. quintana, B. henselae, and C. burnetii, respectively, by the dPCR assay, which matched the results obtained by universal bacterial 16S rRNA gene amplification and sequencing.

* Mailing address: Molecular Infectious Disease Laboratory, Vanderbilt University Hospital, 4605 TVC, Nashville, TN 37232-5310. Phone: (615) 322-0126. Fax: (615) 343-8420. E-mail: yiwei.tang{at}vanderbilt.edu

{triangledown} Published ahead of print on 24 June 2009.

Journal of Clinical Microbiology, August 2009, p. 2647-2650, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.00721-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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