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Journal of Clinical Microbiology, August 2009, p. 2651-2654, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.01946-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Mycobacterium tuberculosis in Ontario, Canada: Insights from IS6110 Restriction Fragment Length Polymorphism and Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat Genotyping{triangledown}

David C. Alexander,1 Jennifer L. Guthrie,1 Daria Pyskir,1 Anne Maki,1 Natalia Kurepina,2 Barry N. Kreiswirth,2 Pamela Chedore,1 Steven J. Drews,1 and Frances Jamieson1*

Ontario Agency for Health Protection and Promotion, Public Health Laboratory—Toronto, 81 Resources Road, Toronto, Ontario, Canada M9P 3T1,1 Public Health Research Institute, Tuberculosis Center, Newark, New Jersey 071032

Received 8 October 2008/ Returned for modification 28 March 2009/ Accepted 26 May 2009

A collection of 1,308 clinical Mycobacterium tuberculosis isolates from Ontario, Canada, was genotyped by IS6110 restriction fragment length polymorphism (RFLP) and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) analysis. RFLP or >12 MIRU-VNTR loci were necessary for resolution of Indo-Oceanic strains. The low clustering rate and high strain diversity indicate that, in Ontario, most tuberculosis results from reactivation of latent infections.

* Corresponding author. Mailing address: Ontario Agency for Health Protection and Promotion, Public Health Laboratory—Toronto, 81 Resources Road, Toronto, Ontario, Canada M9P 3T1. Phone: (416) 235-5841. Fax: (416) 235-5951. E-mail: Frances.Jamieson{at}oahpp.ca

{triangledown} Published ahead of print on 3 June 2009.

Journal of Clinical Microbiology, August 2009, p. 2651-2654, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.01946-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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