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Journal of Clinical Microbiology, August 2009, p. 2662-2666, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.02415-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Use of a New Single Multiplex PCR-Based Assay for Direct Simultaneous Characterization of Six Neisseria meningitidis Serogroups{triangledown}

Christophe Fraisier, Richard Stor, Bernard Tenebray, Yannick Sanson, and Pierre Nicolas*

Unité du Méningocoque, WHO Collaborating Centre, IRBA, Institut de Médecine Tropicale du Service de Santé des Armées, Allée du Médecin Colonel Eugène Jamot, Parc du Pharo, BP 60109, 13262 Marseille Cedex 07, France

Received 17 December 2008/ Returned for modification 26 March 2009/ Accepted 16 June 2009

We developed a new Neisseria meningitidis multiplex PCR to determine six serogroups, including X-specific primers, and to allow direct W135/Y discrimination. This assay offers a simple and low-cost method for serogrouping N. meningitidis from cerebrospinal fluid that could be useful in Africa.

* Corresponding author. Mailing address: Unité du Méningocoque, IMTSSA, Allée du Médecin Colonel Eugène Jamot, Parc du Pharo, BP 60109, 13262 Marseille Cedex 07, France. Phone: 33-4-91-150-115. Fax: 33-4-91-59-44-77. E-mail: meningo{at}imtssa.fr

{triangledown} Published ahead of print on 24 June 2009.

Journal of Clinical Microbiology, August 2009, p. 2662-2666, Vol. 47, No. 8
0095-1137/09/$08.00+0     doi:10.1128/JCM.02415-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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